| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Clostridium neonatale | AGGTGGGGATTTAGCCAATGG | CCACCAATCACAATACCCTCC | 60.06 | 58.35 | 291 | 182 |
100.00%
|
100.00%
|
| Primer 2 | Clostridium neonatale | GGTGGGGATTTAGCCAATGG | CCACCAATCACAATACCCTCC | 58.59 | 58.35 | 290 | 182 |
100.00%
|
100.00%
|
| Primer 3 | Clostridium neonatale | GCTGGTGCAAAGGTCACAG | GCCAATAAAAGGCCAGCTTTC | 59.34 | 58.38 | 171 | 173 |
100.00%
|
100.00%
|
| Primer 4 | Clostridium neonatale | AGCTGGTGCAAAGGTCACAG | GCCAATAAAAGGCCAGCTTTC | 60.82 | 58.38 | 172 | 173 |
100.00%
|
100.00%
|
| Primer 5 | Clostridium neonatale | TAGCTGGTGCAAAGGTCACAG | GCCAATAAAAGGCCAGCTTTC | 60.54 | 58.38 | 173 | 173 |
100.00%
|
100.00%
|
| Primer 6 | Clostridium neonatale | ATAGCTGGTGCAAAGGTCAC | GCCAATAAAAGGCCAGCTTTC | 58.17 | 58.38 | 174 | 169 |
100.00%
|
100.00%
|
| Primer 7 | Clostridium neonatale | ACTGGAGGATGTGGCTGTG | GGCATAACTTGAGCCCATGC | 59.32 | 59.61 | 221 | 168 |
100.00%
|
100.00%
|
| Primer 8 | Clostridium neonatale | AACTGGAGGATGTGGCTGTG | GGCATAACTTGAGCCCATGC | 59.96 | 59.61 | 222 | 168 |
100.00%
|
100.00%
|
| Primer 9 | Clostridium neonatale | ACAACTGGAGGATGTGGCTG | GGCATAACTTGAGCCCATGC | 59.96 | 59.61 | 224 | 168 |
100.00%
|
100.00%
|
| Primer 10 | Clostridium neonatale | TGGCTGTGGAGATCATGACC | GGCATAACTTGAGCCCATGC | 59.46 | 59.61 | 210 | 168 |
100.00%
|
100.00%
|